{"id":338544,"date":"2024-10-19T23:41:30","date_gmt":"2024-10-19T23:41:30","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-15634-22019\/"},"modified":"2024-10-25T22:47:41","modified_gmt":"2024-10-25T22:47:41","slug":"bs-en-15634-22019","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-15634-22019\/","title":{"rendered":"BS EN 15634-2:2019"},"content":{"rendered":"<p>This document specifies a method for the detection of celery (<i>Apium graveolens<\/i>) in emulsion-type sausages (e.g. Frankfurter, Wiener).<\/p>\n<p>Real-time PCR (polymerase chain reaction) detection of celery is based on an 101&nbsp;bp (base pair) sequence from the gene of the mannitol dehydrogenase (GenBank Acc. No. AF067082 <span class=\"xref\"><sup>1)<\/sup><\/span> of celery (<i>Apium graveolens<\/i>).<\/p>\n<p>The method has been validated on emulsion-type sausages (Bavarian \u201cLeberk\u00e4se\u201d) spiked with celery. For this purpose meat batter containing mass fractions of 50&nbsp;% pork meat, 25&nbsp;% pork fat, 23&nbsp;% crushed ice and 1,8&nbsp;% of a mixture of sodium chloride, nitrite, nitrate, phosphates and ascorbates was prepared according to a standard procedure for emulsion-type sausage. The meat batter was spiked with either ground celery seeds or celery root powder to 1000&nbsp;mg\/kg. Lower spiking levels were obtained by diluting with celery-free meat batter. The batter was stuffed into casings and heated at 65&nbsp;\u00b0C for 60&nbsp;min [<span class=\"xref\">1<\/span>].<\/p>\n<p>This document is intended to be used in addition to <span class=\"std\"><span class=\"std-ref\">EN&nbsp;15842<\/span><\/span> and <span class=\"std\"><span class=\"std-ref\">EN&nbsp;15634?1<\/span><\/span>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p><b>Foodstuffs. Detection of food allergens by molecular biological methods &#8211; Celery (Apium graveolens). Detection of a specific DNA sequence in cooked sausages by real-time PCR<\/b><\/p>\n<table class=\"shortdes-table\" style=\"width: 100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Published By<\/td>\n<td>Publication Date<\/td>\n<td>Number of Pages<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/pdfstandards.shop\/product-category\/publishers\/bsi\/\"><b>BSI<\/b><\/a><\/td>\n<td>2019<\/td>\n<td>24<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338547,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[967,2641],"product_tag":[],"class_list":{"0":"post-338544","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-67-120-10","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338544","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338547"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338544"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338544"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338544"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}