{"id":291283,"date":"2024-10-19T19:46:40","date_gmt":"2024-10-19T19:46:40","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-20688-12020\/"},"modified":"2024-10-25T16:48:18","modified_gmt":"2024-10-25T16:48:18","slug":"bs-iso-20688-12020","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-20688-12020\/","title":{"rendered":"BS ISO 20688-1:2020"},"content":{"rendered":"

This document specifies minimum requirements for the production and quality control of synthesized oligonucleotides (nominally up to 250 bases).<\/p>\n

This document also describes general quality attributes for synthesized oligonucleotides as well as common methods for evaluating quality attributes.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
10<\/td>\n4 Design and selection of suitable oligonucleotides that are fit for purpose
5 General quality management requirements
5.1 General requirements <\/td>\n<\/tr>\n
11<\/td>\n5.2 Oligonucleotide grading
5.3 Control of documents
5.4 Quality management system
5.5 Personnel and training
5.6 Safety control
6 \u200bResource management <\/td>\n<\/tr>\n
12<\/td>\n7 Requirements for production process
7.1 General
7.2 Oligonucleotide synthesis
7.3 Purification
7.4 Quality control inspection <\/td>\n<\/tr>\n
13<\/td>\n7.5 Drying
7.6 Formulation
8 Requirements for quality control process
8.1 General
8.2 Validation and verification of analytical methods
8.3 Identity and purity
8.3.1 Identity of the base sequence
8.3.2 Purity <\/td>\n<\/tr>\n
14<\/td>\n8.3.3 Impurity
8.4 Quantity
8.4.1 General
8.4.2 Concentration <\/td>\n<\/tr>\n
15<\/td>\n8.4.3 Mass
8.5 Molar mass and\/or base length <\/td>\n<\/tr>\n
16<\/td>\n8.6 Melting temperature (Tm)
8.7 Report <\/td>\n<\/tr>\n
17<\/td>\n8.8 \u200bVerification of corrective action and improvements
9 Additional requirements for synthetic oligoribonucleotides (RNA) <\/td>\n<\/tr>\n
18<\/td>\nAnnex A (informative) Process checklist <\/td>\n<\/tr>\n
20<\/td>\nAnnex B (informative) List of equipment and devices and their control standards <\/td>\n<\/tr>\n
22<\/td>\nAnnex C (informative) Calculation of molar mass and molar numbers <\/td>\n<\/tr>\n
25<\/td>\nAnnex D (informative) Verification of oligonucleotide sequences using mass analysis <\/td>\n<\/tr>\n
31<\/td>\nAnnex E (informative) Accuracy control of measurement instrument with certified reference materials \u2014 Examples <\/td>\n<\/tr>\n
34<\/td>\nAnnex F (informative) Calculation method of Tm values <\/td>\n<\/tr>\n
36<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Biotechnology. Nucleic acid synthesis – Requirements for the production and quality control of synthesized oligonucleotides<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2020<\/td>\n38<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":291287,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-291283","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/291283","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/291287"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=291283"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=291283"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=291283"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}