ASTM-E2720 2010

$44.96

E2720-10 Standard Test Method for Evaluation of Effectiveness of Decontamination Procedures for Air-Permeable Materials when Challenged with Biological Aerosols Containing Human Pathogenic Viruses

Published By	Publication Date	Number of Pages
ASTM	2010	11

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Categories: 11.080.99 - Other standards related to sterilization and disinfection, ASTM

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Description

1.1 This test method is designed to evaluate decontamination methods (physical, chemical, self-decontaminating materials) when used on air-permeable materials contaminated with virus-containing droplet nuclei.

1.2 This test method defines the conditions for simulating respiratory droplet nuclei produced by humans.

1.3 The method is specific to influenza viruses but could be adapted for work with other types of respiratory viruses or surrogates (Appendix X6).

1.4 This test method is suitable only for air-permeable materials.

1.5 This test method does not address the performance of decontaminants against microbes expelled via blood splatter, vomit, or fecal contamination.

1.6 This test method should be performed only by those trained in bioaerosols, microbiology, or virology, or combinations thereof.

1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

PDF Catalog

PDF Pages	PDF Title
1	Scope Referenced Documents
2	Terminology Summary of Test Method Significance and Use
3	Apparatus Reagents and Materials TABLE 1
4	Sampling, Test Specimens, and Test Units Experimental Design Test Procedure Calculation or Interpretation of Results
5	Report Precision and Bias Keywords X1. EXAMPLE DEVICE: OPERATION OF THE LABORATORY-SCALE AEROSOL TUNNEL (LSAT) X1.1 Diagram of the Laboratory-Scale Aerosol Tunnel (LSAT) FIG. X1.1
6	X1.2 Parameters of the LSAT X1.3 Test Procedure X2. EXAMPLE DEVICE: THE AEROSOL CHAMBER TESTING SYSTEM X2.1 Diagram of the Aerosol Chamber Testing System X2.2 Test Parameters of the Aerosol Chamber Testing System X2.3 Test Procedure
7	FIG. X2.1
8	X3. TCID50 ASSAY FOR INFLUENZA X3.1 Reagents X3.2 Method X4. EXAMPLE CALCULATION FOR TISSUE CULTURE INFECTIOUS DOSE (TCID50) ASSAY X4.1 24-Well Plate X4.2 96-Well Plate FIG. X4.1 FIG. X4.2
9	X5. EXAMPLE CALCULATIONS FOR DATA QUALITY OBJECTIVES AND DECONTAMINATION EFFICIENCY X5.1 Example Data Set X5.2 Average PFU per cm2 X5.3 Data Quality Objectives X5.4 Decontamination Efficiency X6. METHOD ADAPTATION FOR USING BACTERIOPHAGE MS2 X6.1 Scope X6.2 Referenced Documents X6.3 Terminology X6.4 Summary of Test Tethod X6.5 Significance and Use X6.6 Additional Equipment X6.7 Additional Reagents and Materials
10	X6.8 Sampling, Test Specimens, and Test Units X6.9 Experimental Design X6.10 Test Procedure X6.11 Calculation and Interpretation of Results X6.12 Additional Reporting X6.13 Precision and Bias X6.14 Additional Keywords X7. MS2 PLAQUE ASSAY X7.1 Preparation of E. coli (Day 1) X7.2 Inoculation of TSB With E. coli (Day 2) X7.3 Media Preparation and Plating (Day 3) X7.4 Prepare MS2 Soft-Agar Media X7.5 Plating of Samples

Additional information

Standard Title	E2720-10 Standard Test Method for Evaluation of Effectiveness of Decontamination Procedures for Air-Permeable Materials when Challenged with Biological Aerosols Containing Human Pathogenic Viruses
Published Code	ASTM
Publication Date	2010
Pages Count	11
ICS Codes	11.080.99 - Other standards related to sterilization and disinfection

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ASTM-E2720 2010

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