{"id":363618,"date":"2024-10-20T01:46:36","date_gmt":"2024-10-20T01:46:36","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-20397-22021\/"},"modified":"2024-10-26T02:47:11","modified_gmt":"2024-10-26T02:47:11","slug":"bs-iso-20397-22021","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-20397-22021\/","title":{"rendered":"BS ISO 20397-2:2021"},"content":{"rendered":"

This document specifies general requirements and recommendations for quality assessments and control of massively parallel sequencing (MPS) data. It covers post raw data generation procedures, sequencing alignments, and variant calling.<\/p>\n

This document also gives general guidelines for validation and documentation of MPS data.<\/p>\n

This document does not apply to any processes related to de novo assembly.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
14<\/td>\n4 Raw data
4.1 General
4.2 Raw data file
4.3 Quality assessment of raw data
4.3.1 General <\/td>\n<\/tr>\n
15<\/td>\n4.3.2 Basic statistics
4.3.3 Quality metrics <\/td>\n<\/tr>\n
16<\/td>\n4.4 Raw data pre-processing
5 Sequence alignment and mapping
5.1 General <\/td>\n<\/tr>\n
17<\/td>\n5.2 Alignment and mapping file format
5.3 Quality control of sequencing alignment and mapping
5.3.1 Basic alignment statistics <\/td>\n<\/tr>\n
18<\/td>\n5.3.2 Quality indicators <\/td>\n<\/tr>\n
19<\/td>\n5.3.3 Methods for alignment and mapping quality assessment
5.4 Alignment post-processing
6 Variant calling
6.1 General
6.2 Data file for variant calling <\/td>\n<\/tr>\n
20<\/td>\n6.3 Quality metrics in the variant calling
6.4 Processing of false positive variants
6.5 Sequence annotation
7 Validation
7.1 General <\/td>\n<\/tr>\n
21<\/td>\n7.2 Validation of quality metrics <\/td>\n<\/tr>\n
22<\/td>\n8 Documentation <\/td>\n<\/tr>\n
23<\/td>\nAnnex A (informative) Quality metrics for specific example MPS platforms <\/td>\n<\/tr>\n
24<\/td>\nAnnex B (informative) Coverage and read recommendations by applications <\/td>\n<\/tr>\n
26<\/td>\nAnnex C (informative) Software for sequence alignment and mapping <\/td>\n<\/tr>\n
27<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Biotechnology. Massively parallel sequencing – Quality evaluation of sequencing data<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2021<\/td>\n30<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":363625,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-363618","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/363618","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/363625"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=363618"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=363618"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=363618"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}