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BS ISO 23734:2021

BS ISO 23734:2021

$142.49

Marine technology. Marine environment impact assessment (MEIA). On-board bioassay to monitor seawater quality using delayed fluorescence of microalga

Published By Publication Date Number of Pages
BSI 2021 26
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This document specifies a bioassay for the determination of the presence of unknown toxic contaminants in test seawater (see Figure A.1). It is based on the inhibition of photosynthetic activity of the marine cyanobacterium Cyanobium sp. (NIES-981) by such toxic contaminants. The inhibition is determined based on delayed fluorescence (DF) intensity.

The method is rapid and requires less laboratory space than standard bioassays. Hence, it can be used on-board to generate basic data for seawater quality management at deep-sea mining sites where time and space are extremely limited.

PDF Catalog

PDF Pages PDF Title
2 National foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
11 5 Materials
5.1 Test alga
5.2 Reagents
5.2.1 Water
5.2.2 Growth medium
5.2.3 Nutrient, metal and tris stock solutions
12 6 Apparatus
6.1 General
6.2 High sensitivity luminometer
6.3 Incubator and tube shaker
6.4 Apparatus for determining algal cell density
6.5 Culture tubes
6.6 Clean bench
7 Preparations at a land-based laboratory
7.1 Preparation of the growth medium
13 7.2 Preparation of the algal stock culture
8 Test procedure on-board
8.1 Preparation of the algal inoculum culture
8.2 Choice of the test concentrations
8.3 Preparation of the test medium
14 8.4 Inoculation and incubation
9 DF measurement
10 Interpretation of data
10.1 Plotting the DF decay curve
10.2 Calculation of per cent inhibition
15 11 Interpretation of the results
12 Test report
17 Annex A (informative) Schematic overview and procedures of the on-board bioassay
20 Annex B (informative) Preparation of the test medium in seawater
21 Annex C (informative) Practical procedures for the on-board bioassay and schematic diagram of the high sensitivity luminometer
22 Annex D (informative) Cryopreservation procedure
23 Annex E (informative) Reference data for checking the appropriateness of the test procedures
24 Bibliography

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BS ISO 23734:2021
$142.49