BS ISO 20391-1:2018
$142.49
Biotechnology. Cell counting – General guidance on cell counting methods
| Published By | Publication Date | Number of Pages |
| BSI | 2018 | 26 |
This document defines terms related to cell counting for biotechnology. It describes counting of cells in suspension (generally cell concentration) and cells adhered to a substrate (generally area density of cells). It provides key considerations for general counting methods (including total and differential counting, and direct and indirect counting) as well as for method selection, measurement process, and data analysis and reporting.
This document is applicable to the counting of all cell types – mammalian and non-mammalian (e.g. bacteria, yeast) cells.
This document is not intended for counting of cells while in a tissue section or a biomaterial matrix.
Several sector/application-specific international and national standards for cell counting currently exist. When applicable, the user can consult existing standards when operating within their scope (specific measurement techniques and/or applications).
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | National foreword |
| 6 | Foreword |
| 7 | Introduction |
| 9 | 1 Scope 2 Normative references 3 Terms and definitions |
| 12 | 4 General concepts of cell counting 4.1 General |
| 13 | 4.2 Total cell counting 4.3 Differential cell counting 4.4 Direct cell counting 4.5 Indirect cell counting 5 Considerations for cell counting measurements 5.1 Selection of a cell counting method |
| 14 | 5.2 Considerations for selecting a cell counting method 5.3 Sampling of cells for counting |
| 15 | 5.4 Preparation of cell samples for counting 5.4.1 Environmental factors 5.4.2 Procedures 5.4.3 Quality and stability of reagents |
| 16 | 5.5 Performing a measurement 6 Qualification, validation, and verification 6.1 Instrument qualification 6.2 Method validation and verification |
| 17 | 6.3 Reference materials 6.3.1 Certified reference materials 6.3.2 In-house reference materials 6.3.3 Uses of reference materials |
| 18 | 7 Data processing, analysis, and reporting 7.1 Data processing and analysis 7.1.1 General 7.1.2 Image processing and analysis 7.1.3 Gating 7.1.4 Coincidence correction 7.2 Reporting |
| 19 | Annex A (informative) Description of common cell counting methods |
| 22 | Annex B (informative) Common cell counting methods for various measurement purposes |
| 23 | Bibliography |
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ISO 20391-1:2018
Biotechnology — Cell counting — Part 1: General guidance on cell counting methods Published By…
