ASTM-F3504 2021

$34.13

F3504-21 Standard Practice for Quantifying Cell Proliferation in 3D Scaffolds by a Nondestructive Method

Published By	Publication Date	Number of Pages
ASTM	2021

Guaranteed Safe Checkout

Categories: 07.100.99 - Other standards related to microbiology, ASTM

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. We’re here to assist you 24/7.
Email:[email protected]

Description

ASTM F3504-21

Active Standard: Standard Practice for Quantifying Cell Proliferation in 3D Scaffolds by a Nondestructive Method

ASTM F3504

Scope

1.1 This practice describes how to conduct a nondestructive proliferation test for mammalian cells based on metabolic activity that can be used to assess the number of viable cells within three-dimensional (3D) scaffolds for regenerative medicine and in tissue-engineered medical products (TEMPs).

1.2 This practice provides a detailed explanation of the resazurin cell metabolic activity method in terms of reagent concentrations, incubation times, cell culture media composition, calibration curve, controls, assay linearity, and limitations of the assay.

1.3 This practice describes factors that can interfere with accurate cell proliferation assessment.

1.4 Since the assay has washing steps, it is limited to assessing cells that are immobilized, such as by adhesion to a culture dish, adhesion to a scaffold, or encapsulation in a hydrogel.

1.5 The assay is limited to cell types that can metabolize resazurin to provide a signal in the assay.

1.6 This document does not propose acceptance criteria for a cell-based product based on the application of a cell proliferation test method.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Keywords

cell counting; cell number; cell proliferation; resazurin; stem cells; tissue regeneration;

ICS Code

ICS Number Code 07.100.99 (Other standards related to microbiology)

DOI: 10.1520/F3504-21

Additional information

Standard Title	F3504-21 Standard Practice for Quantifying Cell Proliferation in 3D Scaffolds by a Nondestructive Method
Published Code	ASTM
Publication Date	2021
ICS Codes	07.100.99 - Other standards related to microbiology